Open Access

Q&A: what are brassinosteroids and how do they act in plants?

BMC Biology201614:113

DOI: 10.1186/s12915-016-0340-8

Published: 22 December 2016

Abstract

Brassinosteroids (BRs) are a class of polyhydroxylated steroidal phytohormones in plants with similar structures to animals’ steroid hormones. Brassinosteroids regulate a wide range of physiological processes including plant growth, development and immunity. Brassinosteroid signalling and its integration with other signalling pathways have been investigated thoroughly at the molecular level.

What are the plant steroid hormones brassinosteroids?

Brasinosteroids, defined as the sixth plant hormone after the classic plant hormones auxin, gibberellins, cytokinin, abscisic acid and ethylene, are analogous to animal steroid hormones in structure [1, 2]. Similar to animal hormones, brassinosteroids play crucial roles in diverse aspects of plant biology, including cell elongation, cell division, root growth, photo-morphogenesis, stomatal and vascular differentiation, seed germination, immunity and reproduction [3, 4]. Brassinosteroids are also involved in regulating the metabolism of plant oxidation radicals, ethylene synthesis and root gravitropic response, and have a role in mediating plant responses to stress, such as freezing, drought, salinity, disease, heat and nutrient deficiency [57]. This subfamily of hormones regulates a broad range of processes in plant development and responses to environmental stresses, and their analogs have been shown to bring substantial increases in grain yield, depending on growth status.

How were brassinosteroids found?

Mitchell and co-workers extracted specific ingredients with growth-promoting activity from Brassica pollen after screening nearly 60 species of plants. Histological analysis showed that the reactions induced by these ingredients were different from those induced by gibberellins [8]. They therefore speculated that these ingredients were a new class of hormones, termed brassins. This hypothesis, however, was not accepted by some other researchers, who argued that the physiological activities of the ingredients could have been caused by gibberellin due to the crude nature of the extract from which brassins were identified [9]. Given the potential applications of brassins in agriculture, efforts organized by the US Department of Agriculture led to purification of 4 mg of brassins from 500 pounds of bee-collected Brassica pollen. The crystal structure of the purified brassins was then solved and brassinolide was identified as the active component [10]. These findings marked the discovery of the first plant steroidal hormone. Currently, nearly 70 kinds of natural brassinolide analogues have been isolated from tissues of various plant species, which compose the new class of plant hormone, BRs [11].

What are the structures of brassinosteroids?

Brassinosteroids are classified as C27, C28 or C29 based on different alkyl-substitution patterns of the side chains. In general, a trans-fused A/B ring system with two hydroxyl groups at ring A and a 6-ketone or 7-oxa-6-ketone system at ring B are required for active BRs [1]. The chemical structure of brassinolide ((22R, 23R, 24S)-2a, 3a, 22, 23-tetrahydroxy-24-methyl-B-homo-7-oxa-5a-cholestan-6-one) [10], the most active BR identified thus far, is shown in Fig. 1. Other BRs differ from BL within the boxed area (a) and (b) on the basis of a 5α-cholestane skeleton [12] (Fig. 1). Additionally, BR conjugated forms with sugars or fatty acids have also been found, which are the inactive products of BR metabolism [12].
Fig. 1

The chemical structure of brassinolide (BL) with the steroid rings labelled as A, B, C and D. The parts within the dashed lines can be substituted by different groups, as described in the text

What parts of plants are brassinosteroids usually found in?

Brassinosteroids have been found in various species of plants, including monoplast freshwater algae and brown algae, indicating that BRs are a widespread ancient plant hormone [11]. Distributions of BRs differ among distinct tissues of individual species. Pollens, immature seeds, roots and flowers were found to have the highest content, ranging from 1–100 ng/g fw (fresh weight), while shoots and leaves have lower amounts 0.01–0.1 ng/g fw [13]. The content and distributions of different BR analogues also vary among tissues. Unlike other hormones, endogenous BRs do not move between tissues but function in a paracrine or autocrine way as demonstrated by grafting experiments using a BR-deficient mutant of pea [14, 15]. One reason for this is likely that BR biosynthesis genes are widespread in various tissues of plant and BRs can be synthesized in situ. Long-distance effects of BRs depend on their crosstalk with other hormones like auxins and gibberellin [16]. However, BRs need transporting from their synthesis sites in the ER to the plasma membrane and early endosomal compartments where they are perceived through passive or active intracellular transport.

What do plants do with excess brassinosteroids?

Plants synthesize BRs continuously to meet their need for growth and development, but excess BRs can be metabolized rapidly, as demonstrated by exogenous application of BRs. BR metabolism can be classified into modification of their steroidal skeletons and modification of their side chains [17]. A number of reactions, such as dehydrogenation, demethylation, epimerization, esterification, glycosylation, hydroxylation, side-chain cleavage and sulfonation, have been found to inactivate BRs, though the mechanisms underlying this remain unclear. It has been suggested that inactive BRs can be converted into active forms to maintain BR homeostasis [12, 18].

What are the receptors for brassinosteroids?

Studies from several laboratories contributed to the finding of the first BR receptor [19]. Clouse et al. identified the first BR-insensitive (BRI) mutant (named bri1) by observing the promotion of root elongation under inhibitory concentrations of BR compared to the wild type in Arabidopsis [20]. The bri1 mutant displayed dwarfism, reduced cell elongation, dark-green and thickened leaves, reduced apical dominance, delayed blooming and senescence, altered vascular patterning and male sterility. The positional cloning of BRI1 was performed by Jianming Li and J. Chory, who identified 18 alleles of bri1. Despite structural similarity between BRs and animal steroid hormones, BRI1 does not structurally resemble the nuclear steroid receptors of animals but encodes a leucine-rich repeat receptor-like kinase (LRR-RLK) with an extracellular leucine-rich repeat (LRR) domain and an intracellular serine/threonine kinase domain [21]. BRI1 is highly conserved across different plant species [19], consistent with the finding that BRs are widely present in plants. There are three BRI1 homologues in Arabidopsis, BRL1, BRL2 and BRL3. BRL1 and BRL3, but not BRL2, were shown to bind BRs with high affinity and rescue the phenotypes of the BRI1 mutation when expressed using the promoter of BRI1 [22]. Thus far, the ligands BRL2 might recognize still remain unknown. BRI1 is highly expressed in various tissues of plants and functions as the major receptor of BRs, whereas the expression of BRL1 and BRL3 is confined to vascular cells and display weak phenotypes when knocked out [22].

What are the BRI1-associated receptor kinases?

In 2002, J.C. Walker’s group, using a bri1 suppressor screen, and Jianming Li’s group, using a yeast two-hybrid screen, independently found a BRI1-interacting partner, an LRR-RLK named BRI1-associated kinase 1 (BAK1). Both groups presented evidence showing that BRI1 and BAK1 interacted with each other in vitro and in vivo, which contributed to BR signalling [23, 24]. BAK1 is also called SERK3, as it belongs to the family of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASEs (SERKs), of which there are five members (SERK1–SERK5) in Arabidopsis [25]. Later on, SERK1 and SERK4 were shown to have a similar role to BAK1/SERK3 in BR-induced signalling [26, 27]. The serk1 bak1 bkk1 triple null mutant phenocopied a null bri1 mutant, establishing an indispensable role of SERKs in BR signalling [27].

How does BRI1 recognize brassinosteroids and consequently become activated?

In 2011, two groups provided structural and biochemical data demonstrating that the extracellular LRR domain of BRI1 (BRI1LRR) was sufficient for recognition of BL [28, 29]. Structural analyses revealed that a hydrophobic groove, formed between the inner surface of the helical BRI1LRR and an embedded island domain, is responsible for the specific recognition of BL by BRI1 (Fig. 2). This mechanism is conserved in the recognition of BL by BRL1 [30] and likely BRL3 given the highly conserved amino acids of the BL-binding groove among the three BR receptors. Two years later, the same two groups presented the crystal structures of BRI1LRR-BL-BAK1LRR/SERK1LRR ternary complexes [31, 32]. Coupled with biochemical data, these structures demonstrated that BL induced a heterodimeric BRI1LRR-BAK1/SERK1LRR complex for BRI1 activation (Fig. 2). These results indicate that ligand-induced dimerization seen for the activation of receptor tyrosine kinases (RTKs) and other single trans-membrane receptors in animals is also conserved in the activation of plant RLKs [3336]. Furthermore, BAK1 or SERK1 in the complex is directly involved in recognition of BL, forming one heterodimerization interface between BRI1LRR and BAK1LRR/SERK1LRR [31, 32]. These results indicate that BAK1/SERK1 function as co-receptors with BRI1 for BR-induced BRI1 activation.
Fig. 2

Brassinolide induces BRI1 heterodimerization with SERKs. a Overall structure of BL bound by BRI1LRR (green) shown as a structure graphic. BL (pink) is shown as a stick diagram. The island domain of BRI1 is shown in grey. ‘N’ and ‘C’ represent the N-terminal and C-terminal side of BRI1LRR, respectively. b Crystal structure of BRI1LRR-BL-BAK1LRR complex. BAK1LRR is shown in pale green

How are the signals downstream of brassinosteroid signalling transduced?

The BR signalling pathway is a complex phosphor-relay system that mediates plant growth and development [37] (Fig. 3). BR binding induces BRI1 phosphorylation of BRI1-KINASE INHIBITOR 1 (BKI1), thus relieving BKI1 inhibition of BRI1 and fully activating BRI1 through mutual phosphorylation between BRI1 and BAK1 or other SERK members. Once activated, BRI1 phosphorylates the BR-SIGNALING KINASE (BSK1) and CONSTITUTIVE DIFFERENTIAL GROWTH 1 (CDG1) kinase, which in turn activate a PP1-type phosphatase named BRI1-SUPPRESSOR 1 (BSU1). The activated BSU1 and PROTEIN PHOSPHATASE 2A (PP2A) dephosphorylate and inactivate the GSK3-like kinase named BRASSINOSTEROID INSENSITIVE 2 (BIN2), allowing BR response transcription factors BRASSINAZOLE RESISTANT 1/2 (BZR1/2) to accumulate in the nucleus and bind to DNA. Additionally, many other transcription factors also contribute to the global BR responses [3840].
Fig. 3

The brassinosteroid signal transduction pathway in the absence (a) and presence (b) of brassinosteroid (BR) in Arabidopsis. Brassinosteroid binding induces BRI1 heterodimerization with its co-receptor BAK1, resulting in activation of BRI1, phosphorylation of BKI1 by BRI1 and dissociation of BKI1 from BRI1. These events lead to full activation of BRI1, which phosphorylates BSK1 and CDG1. Once activated, BSK1 and CDG1 promote the activation of the phosphatase BSU1, thus dephosphorylating and inactivating BIN2. The inactivated BIN2 allows the presence of the dephosphorylated form of BZR1/2, which can move into the nucleus and regulate transcription of many target genes. The phosphorylated BZR1/2 are retained in the cytoplasm by 14-3-3. Arrows and short lines represent promotion and inhibition, respectively. The small circles containing the letter ‘P’ indicate phosphorylation

The potential role of brassinosteroids in the plant growth-immunity trade-off is intriguing—can you describe it?

Brassinosteroids regulate many development processes in plants, as well as responses to environmental stresses and their roles in the growth–defence trade-off have profound implications in agriculture and natural ecosystems. To ensure perpetuation, plants need to balance their limited resources for growth and defence [41]. Several plant hormones, including BRs, have been suggested to play roles in the trade-off between growth and defence [42]. Recent studies indicated that interaction of BR signalling with PAMP-triggered immunity is unidirectional and negative [43, 44]. More recent data appear to support the idea that the interaction is located at the transcriptional level rather than at the receptor complex [4548], though the underlying mechanisms remain debatable. Understanding of the mechanisms for these trade-offs is expected to provide a foundation for development of breeding strategies to maximize crop yield.

What other unknowns are there in brassinosteroid signalling?

Great advances have been achieved in our understanding of the BR signalling pathway and BR biosynthesis and metabolism. However, it remains unclear how brassinosteroid-induced BRI1-BAK1 heterodimerization activates the kinase activity of BRI1. Fully addressing this question will require detailed elucidation of structure of the full-length complex. New components are being identified in brassinosteroid signalling and understanding of how they are integrated with other signalling pathways will be important to gain a comprehensive and systematic view of brassinosteroid functions in various plants [49, 50]. A full elucidation of brassinosteroid signalling events and their integration with other signalling pathways will enable brassinosteroid to be applied in agriculture.

Declarations

Acknowledgements

JC is supported by grants from Projects of International Cooperation and Exchanges NSFC (31420103906), National Natural Science Foundation of China (31130063 and 31421001), and Chinese Ministry of Science and Technology (2015CB910200).

Competing interests

The authors declare that they have no competing interests.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Key Laboratory of Protein Sciences (Tsinghua University), Ministry of Education
(2)
Tsinghua-Peking Center for Life Sciences
(3)
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences

References

  1. Taiz L, Zeiger E. Plant Physiol. 5th ed. Sunderland: Sinauer Associates; 2010.Google Scholar
  2. Clouse SD. Brassinosteroids. The Arabidopsis book. 2011;9:e0151.View ArticlePubMedPubMed CentralGoogle Scholar
  3. Gudesblat GE, Russinova E. Plants grow on brassinosteroids. Curr Opin Plant Biol. 2011;14:530–7.View ArticlePubMedGoogle Scholar
  4. Wei Z, Li J. Brassinosteroids regulate root growth, development, and symbiosis. Mol Plant. 2016;9:86–100.View ArticlePubMedGoogle Scholar
  5. Krishna P. Brassinosteroid-mediated stress responses. J Plant Growth Regulation. 2003;22:289–97.View ArticleGoogle Scholar
  6. Ashraf M, Akram NA, Arteca RN, Foolad MR. The physiological, biochemical and molecular roles of brassinosteroids and salicylic acid in plant processes and salt tolerance. Crit Rev Plant Sci. 2010;29:162–90.View ArticleGoogle Scholar
  7. Bajguz A, Hayat S. Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiol Biochem. 2009;47:1–8.View ArticlePubMedGoogle Scholar
  8. Mitchell J, Mandava N, Worley J, Plimmer J, Smith M. Brassins-a new family of plant hormones from rape pollen. Nature. 1970;225:1065–6.View ArticlePubMedGoogle Scholar
  9. Milborrow BV, Pryce RJ. The brassins. Nature. 1973;243:46.View ArticleGoogle Scholar
  10. Grove MD, Spencer GF, Rohwedder WK, Mandava N, Worley JF, Warthen JD, Steffens GL, Flippen-Anderson JL, Cook JC. Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen. Nature. 1979;281:216–7.View ArticleGoogle Scholar
  11. Kutschera U, Wang ZY. Brassinosteroid action in flowering plants: a Darwinian perspective. J Exp Bot. 2012;63:3511–22.View ArticlePubMedPubMed CentralGoogle Scholar
  12. Fujioka S, Yokota T. Biosynthesis and metabolism of brassinosteroids. Annu Rev Plant Biol. 2003;54:137–64.View ArticlePubMedGoogle Scholar
  13. Takatsuto S. Brassinosteroids: distribution in plants, bioassays and microanalysts by gas chromatography–mass spectrometry. J Chromatogr A. 1994;658:3–15.View ArticleGoogle Scholar
  14. Symons GM, Ross JJ, Jager CE, Reid JB. Brassinosteroid transport. J Exp Bot. 2008;59:17–24.View ArticlePubMedGoogle Scholar
  15. Bishop GJ, Yokota T. Plants steroid hormones, brassinosteroids: current highlights of molecular aspects on their synthesis/metabolism, transport, perception and response. Plant Cell Physiol. 2001;42:114–20.View ArticlePubMedGoogle Scholar
  16. Lacombe B, Achard P. Long-distance transport of phytohormones through the plant vascular system. Curr Opin Plant Biol. 2016;34:1–8.View ArticlePubMedGoogle Scholar
  17. Bajguz A. Metabolism of brassinosteroids in plants. Plant Physiol Biochem. 2007;45:95–107.View ArticlePubMedGoogle Scholar
  18. Vriet C, Russinova E, Reuzeau C. From squalene to brassinolide: the steroid metabolic and signaling pathways across the plant kingdom. Mol Plant. 2013;6:1738–57.View ArticlePubMedGoogle Scholar
  19. Clouse SD. A history of brassinosteroid research from 1970 through 2005: thirty-five years of phytochemistry, physiology, genes, and mutants. J Plant Growth Regulation. 2015;34:828–44.View ArticleGoogle Scholar
  20. Clouse SD, Langford M, McMorris TC. A brassinosteroid-insensitive mutant in Arabidopsis thaliana exhibits multiple defects in growth and development. Plant Physiol. 1996;111:671–8.View ArticlePubMedPubMed CentralGoogle Scholar
  21. Li J, Chory J. A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell. 1997;90:929–38.View ArticlePubMedGoogle Scholar
  22. Cano-Delgado A, Yin Y, Yu C, Vafeados D, Mora-Garcia S, Cheng JC, Nam KH, Li J, Chory J. BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis. Development. 2004;131:5341–51.View ArticlePubMedGoogle Scholar
  23. Li J, Wen J, Lease KA, Doke JT, Tax FE, Walker JC. BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. Cell. 2002;110:213–22.View ArticlePubMedGoogle Scholar
  24. Nam KH, Li J. BRI1/BAK1, a receptor kinase pair mediating brassinosteroid signaling. Cell. 2002;110:203–12.View ArticlePubMedGoogle Scholar
  25. Hecht V, Vielle-Calzada J-P, Hartog MV, Schmidt ED, Boutilier K, Grossniklaus U, de Vries SC. The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol. 2001;127:803–16.View ArticlePubMedPubMed CentralGoogle Scholar
  26. Karlova R, Boeren S, Russinova E, Aker J, Vervoort J, de Vries S. The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 protein complex includes BRASSINOSTEROID-INSENSITIVE1. Plant Cell. 2006;18:626–38.View ArticlePubMedPubMed CentralGoogle Scholar
  27. Gou X, Yin H, He K, Du J, Yi J, Xu S, Lin H, Clouse SD, Li J. Genetic evidence for an indispensable role of somatic embryogenesis receptor kinases in brassinosteroid signaling. PLoS Genet. 2012;8:e1002452.View ArticlePubMedPubMed CentralGoogle Scholar
  28. Hothorn M, Belkhadir Y, Dreux M, Dabi T, Noel JP, Wilson IA, Chory J. Structural basis of steroid hormone perception by the receptor kinase BRI1. Nature. 2011;474:467–71.View ArticlePubMedPubMed CentralGoogle Scholar
  29. She J, Han Z, Kim TW, Wang J, Cheng W, Chang J, Shi S, Wang J, Yang M, Wang ZY, et al. Structural insight into brassinosteroid perception by BRI1. Nature. 2011;474:472–6.View ArticlePubMedPubMed CentralGoogle Scholar
  30. She J, Han Z, Zhou B, Chai J. Structural basis for differential recognition of brassinolide by its receptors. Protein Cell. 2013;4:475–82.View ArticlePubMedPubMed CentralGoogle Scholar
  31. Santiago J, Henzler C, Hothorn M. Molecular mechanism for plant steroid receptor activation by somatic embryogenesis co-receptor kinases. Science. 2013;341:889–92.View ArticlePubMedGoogle Scholar
  32. Sun Y, Han Z, Tang J, Hu Z, Chai C, Zhou B, Chai J. Structure reveals that BAK1 as a co-receptor recognizes the BRI1-bound brassinolide. Cell Res. 2013;23:1326–9.View ArticlePubMedPubMed CentralGoogle Scholar
  33. Lemmon MA, Schlessinger J. Cell signaling by receptor tyrosine kinases. Cell. 2010;141:1117–34.View ArticlePubMedPubMed CentralGoogle Scholar
  34. Han Z, Sun Y, Chai J. Structural insight into the activation of plant receptor kinases. Curr Opin Plant Biol. 2014;20:55–63.View ArticlePubMedGoogle Scholar
  35. Song W, Han Z, Wang J, Lin G, Chai J. Structural insights into ligand recognition and activation of plant receptor kinases. Curr Opin Struct Biol. 2016;43:18–27.View ArticlePubMedGoogle Scholar
  36. Ma R, Han Z, Hu Z, Lin G, Gong X, Zhang H, Nasrallah JB, Chai J. Structural basis for specific self-incompatibility response in Brassica. Cell Res. 2016;26:1320–29.View ArticlePubMedGoogle Scholar
  37. Clouse SD. Brassinosteroid signal transduction: from receptor kinase activation to transcriptional networks regulating plant development. Plant Cell. 2011;23:1219–30.View ArticlePubMedPubMed CentralGoogle Scholar
  38. Yin Y, Vafeados D, Tao Y, Yoshida S, Asami T, Chory J. A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis. Cell. 2005;120:249–59.View ArticlePubMedGoogle Scholar
  39. Yu X, Li L, Li L, Guo M, Chory J, Yin Y. Modulation of brassinosteroid-regulated gene expression by Jumonji domain-containing proteins ELF6 and REF6 in Arabidopsis. Proc Natl Acad Sci U S A. 2008;105:7618–23.View ArticlePubMedPubMed CentralGoogle Scholar
  40. Sun Y, Fan XY, Cao DM, Tang W, He K, Zhu JY, He JX, Bai MY, Zhu S, Oh E, et al. Integration of brassinosteroid signal transduction with the transcription network for plant growth regulation in Arabidopsis. Dev Cell. 2010;19:765–77.View ArticlePubMedPubMed CentralGoogle Scholar
  41. Huot B, Yao J, Montgomery BL, He SY. Growth-defense tradeoffs in plants: a balancing act to optimize fitness. Mol Plant. 2014;7:1267–87.View ArticlePubMedPubMed CentralGoogle Scholar
  42. Lozano-Duran R, Zipfel C. Trade-off between growth and immunity: role of brassinosteroids. Trends Plant Sci. 2015;20:12–9.View ArticlePubMedGoogle Scholar
  43. Albrecht C, Boutrot F, Segonzac C, Schwessinger B, Gimenez-Ibanez S, Chinchilla D, Rathjen JP, de Vries SC, Zipfel C. Brassinosteroids inhibit pathogen-associated molecular pattern-triggered immune signaling independent of the receptor kinase BAK1. Proc Natl Acad Sci U S A. 2012;109:303–8.View ArticlePubMedGoogle Scholar
  44. Belkhadir Y, Jaillais Y, Epple P, Balsemao-Pires E, Dangl JL, Chory J. Brassinosteroids modulate the efficiency of plant immune responses to microbe-associated molecular patterns. Proc Natl Acad Sci U S A. 2012;109:297–302.View ArticlePubMedGoogle Scholar
  45. Lozano-Durán R, Macho AP, Boutrot F, Segonzac C, Somssich IE, Zipfel C. The transcriptional regulator BZR1 mediates trade-off between plant innate immunity and growth. Elife. 2013;2:e00983.View ArticlePubMedPubMed CentralGoogle Scholar
  46. Bai MY, Fan M, Oh E, Wang ZY. A triple helix-loop-helix/basic helix-loop-helix cascade controls cell elongation downstream of multiple hormonal and environmental signaling pathways in Arabidopsis. Plant Cell. 2012;24:4917–29.View ArticlePubMedPubMed CentralGoogle Scholar
  47. Fan M, Bai MY, Kim JG, Wang T, Oh E, Chen L, Park CH, Son SH, Kim SK, Mudgett MB, et al. The bHLH transcription factor HBI1 mediates the trade-off between growth and pathogen-associated molecular pattern-triggered immunity in Arabidopsis. Plant Cell. 2014;26:828–41.View ArticlePubMedPubMed CentralGoogle Scholar
  48. Malinovsky FG, Batoux M, Schwessinger B, Youn JH, Stransfeld L, Win J, Kim SK, Zipfel C. Antagonistic regulation of growth and immunity by the Arabidopsis basic helix-loop-helix transcription factor homolog of brassinosteroid enhanced expression2 interacting with increased leaf inclination1 binding bHLH1. Plant Physiol. 2014;164:1443–55.View ArticlePubMedPubMed CentralGoogle Scholar
  49. Chaiwanon J, Garcia VJ, Cartwright H, Sun Y, Wang ZY. Immunophilin-like FKBP42/TWISTED DWARF1 interacts with the receptor kinase BRI1 to regulate brassinosteroid signaling in Arabidopsis. Mol Plant. 2016;9:593–600.View ArticlePubMedGoogle Scholar
  50. Zhao B, Lv M, Feng Z, Campbell T, Liscum E, Li J. TWISTED DWARF 1 associates with BRASSINOSTEROID-INSENSITIVE 1 to regulate early events of the brassinosteroid signaling pathway. Mol Plant. 2016;9:582–92.View ArticlePubMedGoogle Scholar

Copyright

© The Author(s). 2016