GFP::TbAGO1 is localised in the cytoplasm. Live cells from cell line TbAGO1-/- +GFP::TbAGO1
Tiwere observed by phase contrast (top panels) or by direct GFP fluorescence (bottom panels, images were not digitally enhanced). The position in the cell cycle can be evaluated by the number and position of flagellar pockets (indicated by yellow stars) and the distance separating them. Arrows indicate the position of the nucleolus (dark spot within the nucleus). A. Cell with one kinetoplast and one nucleus. B, C. Cells with two kinetoplasts and one mitotic nucleus. D. Post-mitotic cell with two kinetoplasts and two nuclei.