Figure 2From: Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stressSecretory perturbations increase oxygen and ATP consumption. (a) Flux balance analysis of strains. TCA cycle is suppressed in high glucose. Bar graphs show flux in mmol/gDCW/h. Complete fluxes in Additional file 4. FBA calculations were performed using n = 3 measurements with errors noted in Additional file 1 (Tables S1 and S2). The average coefficient of variance for these measurements is 11%. (b) Oxygen required for growth. The amount of oxygen consumed for each gram of cells (DCW) formed. Oxygen measurements are mean +/- s.e.m. (n = 3). (c) Specific maintenance ATP consumption as calculated by flux balance analysis (see Materials and Methods for details of calculation). FBA calculations were performed using n = 3 measurements with errors noted in Additional file 1 (Table S1 and S2). The average coefficient of variance for these measurements is 11%. Wild-type, no protein secretion (WN), wild-type insulin precursor secretion (WI), wild-type α-amylase secretion (WA), Δhac1 no protein secretion (dN), Δhac1 insulin precursor secretion (dI), Δhac1 α-amylase secretion (dA). Gram dry cell weight (gDCW). acetyl-CoA (Ac-CoA); fructose-6-phosphate (F6P); glyceraldehyde-3-phosphate (G3P); glucose-6-phosphate (G6P); pyruvate (PYR).Back to article page