Figure 4
Expression of CiTH and of Ci-ADRα2a receptor in the neuronal network of C. intestinalis larva. Confocal sections of larvae co-electroporated with pCi-TH-WGA and constructs reporting expression of the following neurotransmitter markers: ( a, d ) glutamate transporter (pCi-vGluT-eGFP), ( b, e ) GABA transporter (pCi-vGaT-eGFP) and ( c, f ) acetylcholine transporter (pCi-VAchT-eGFP). Localizations of WGA (red) and eGFP (green) were visualized by immunofluorescent staining with an anti-WGA antibody and an anti-GFP antibody, respectively. Merged images (a, b, c) corresponds to merged images with Nomarski channel (d, e, f). A, anterior, P, posterior. (Scale bar, 25 μm in (a, b), 10 μm in c.). (c-n) Confocal sections of larvae with co-electroporated pCi-ADRα2a-Venus and constructs reporting expression of the following markers of neurotransmitter systems: (c, d, e) dopamine (pCiTH-Kaede), (f, g, h) glutamate (pCi-vGluT-Kaede), (i, j, k) GABA (pCi-vGaT-Kaede) and (l, m, n) acetylcholine (pCi-VAchT-Kaede). Localization of Venus (green) (c, f, i, l) Kaede (red) (d, g, j, m) were respectively visualized by immunofluorescent staining with an anti-Kaede antibody and an anti-GFP antibody. (d, h, k, n) merged images of anti-Kaede and anti-Venus staining. These are representative example of all the performed experiments (n = 5 to 15) depending on the couple of transgenes used.