Mating initiation times and re-mating propensity of OX3097D-Bol with wild olive flies. (A) Copulation initiation times were similar for OX3097D-Bol males and wild males. Copulation initiation times were recorded for all mating pairs; each pair contained a wild female and either an OX3097D-Bol male (left circle, n = 216) or a wild male (right circle, n = 161). Scotophase is the dark phase of a light/dark cycle. Each 'wedge' on the circular graphic represents a time-interval of 45 minutes; the radial length of the wedge indicates the proportion of total matings of that type that occurred in each time segment. Mean copulation initiation time for wild females and either OX3097D-Bol or wild males was 63 and 66 minutes before scotophase respectively. Peak mating activity times were not significantly different between the two types of male (P = 0.45, degrees of freedom (d.f.) = 1, circular statistics F-test). (B) Genotype of first mate (OX3097D-Bol or wild) did not affect female re-mating frequency or genotype of second mate. Of 188 females initially mated to OX3097D-Bol males 32 (17%) re-mated, of which 17 (9%) re-mated to wild males (open portion of left bar), and 13 (8%) to OX3097D-Bol males (solid portion of left bar). Of 296 females initially mated to wild males 55 (19%) re-mated, of which 23 (8%) re-mated to wild males (open portion of right bar) and 32 (11%) to OX3097D-Bol males (solid portion of left bar). Re-mating propensity of wild females initially mated with either an OX3097D-Bol male or a wild male were not significantly different (P = 0.7, d.f. = 1, χ2 test). Furthermore, the re-mating preference of the wild females was not found to differ significantly depending on first-mate choice (P = 0.38, d.f. = 1, χ2 test).