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Table 1 buffy mutant larvae maintain smaller steady-state lipid and glycogen stores

From: Drosophila larvae lacking the bcl-2 gene, buffy, are sensitive to nutrient stress, maintain increased basal target of rapamycin (Tor) signaling and exhibit characteristics of altered basal energy metabolism

  Wild-type buffy H37 buffy H37 /wt
Complete medium:    
Nile Red fat body (mean luminosity) 62.4 ± 3.6 (3) 50.4 ± 2.5 (3) 0.81
Nile Red lysate (FU) 323 ± 47 (4) 260 ± 16 (4) 0.80
Triacylglyceride (μg TAG/μg protein) 0.56 ± 0.05 (4) 0.47 ± 0.04 (4) 0.85
Glycogen (ng glycogen/μg protein) 1.54 ± 0.16 (6) 1.30 ± 0.11 (5) 0.85
20% medium:    
Nile Red fat body (mean luminosity) 62.2 ± 15.5 (3) 46.3 ± 1.6 (3) 0.74
Number of lipid droplets/fat body cell 30.7 ± 5.3 (3) 20.7 ± 3.4 (3) 0.67
Perimeter of lipid droplets 38.9 ± 3.4 (3) 32.3 ± 2.2 (3) 0.83
Complete medium + added sucrose:    
Triacylglyceride (μg TAG/μg protein) 0.87 ± 0.07 (4) 0.74 ± 0.10 (4) 0.85
  1. All experiments were performed on third instar larvae fed the indicated medium. All data are presented as averages ± SEM with number of biological replicates in parentheses. With the exception of mean luminosity of Nile Red fat body in complete medium (P = 0.05), all other differences are not significant (P > 0.1) as determined by Student's unpaired two-tailed t test. Nevertheless, the trend is similar across all measurements. For TAG assays, a representative of four biological replicates is presented. For technical reasons the mean luminosity cannot be compared across different media conditions (see Methods for experimental details).