Table 1 E. coli strains
From: Excessive folate synthesis limits lifespan in the C. elegans: E. coliaging model
Strains | Genotype/Relevant characteristics | Source |
|---|---|---|
OP50 | OP50 ura | [37] |
RNAi control strain | HT115(DE3) W3110 rnc14::Tn10 (DE3 PlacUV5-T7 polymerase) | [46] |
aroD mutant | HT115(DE3) aroD717::IS1 | This study |
Keio collection WT | BW25113 | [16] |
Keio collection aroD | BW25113 aroD | |
OP50 Su resistant | OP50 R26 | This study; [47] |
aroD mutant/ ugt-27 RNAi | HT115(DE3)aroD717::IS1/L4440(ugt-27) | This study; [38] |
RNAi control + vector | HT115(DE3)/pMMB67EH | This study |
aroD mutant + vector | HT115(DE3)aroD717::IS1/pMMB67EH | This study |
mutant + aroD | HT115(DE3)aroD717::IS1/pMMB67EH[aroD] | This study |
ubiG mutant | GD1 ubiG | [15] |
ubiG mutant + rescue | GD1 ubiG/pAHG(ubiG+) | [15] |