TWIST1 and IL8 expression is enriched in the basal subtype of human breast cancers. (A) Reverse-transcription PCR of indicated cell lines for TWIST1 and IL8 with β-ACTIN as control. (B) Relative mRNA levels of IL8 and TWIST1 of luminal A (LumA), ERBB2/HER2+ (ERBB2) and basal subtypes from STOCKHOLM human breast tumor dataset (total n = 159). Boxes represent the lower quartile, median and upper quartile; Whiskers represent the tenth and ninetieth percentiles; and dots represent outliers. P-values were calculated using Mann-Whitney U test. (C) Relative mRNA levels of IL8 and TWIST1 of LumA, ERBB2 and basal subtypes from UPPSALA human breast tumor dataset (total n = 253). Graphs were plotted and P-values calculated as described in (B).