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Figure 1 | BMC Biology

Figure 1

From: Erratum to: A novel deconvolution method for modeling UDP-N-acetyl-D-glucosaminebiosynthetic pathways based on 13C mass isotopologue profiles undernon-steady-state conditions

Figure 1

Species assignments of UDP- N -acetyl-D-glucosamine (UDP-GlcNAc)isotopologues in Fourier transform-ion cyclotron resonance-mass spectrometry(FT-ICR-MS). The same crude extracts used for NMR were analyzed followingre-exchange of 2H back to 1H. Analysis conditions are statedin the text. With correction to an internal reference, all of the isotopologueswere assignable at better than 1 ppm mass accuracy, with most better than 10 ppbmass accuracy. The molecular formulae were assigned using Xcalibur software withelemental limits set to CHONP and allowing up to 17 occurrences of 13C.The combination of the ultra-high resolution with extreme mass accuracy resultedin high confidence that only 'pure' 13C isotopologues were quantifiedfor the moiety modeling.

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BMC Biology

ISSN: 1741-7007

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