Figure 3

Change in behavioral features as a function of thermal pulse of increasing amplitude. (A-G) Boxplots of the corresponding behavioral feature plotted for ΔT = 0.4°C (blue), 1.0°C (orange), 4.8°C (red) and 9.1°C (green) for N2 animals. The black lines depict the trend with increasing temperature. The grey shading represents the estimated pointwise 95% confidence intervals. (A-C) Behavioral states (depicted in the ethogram of Figure 2B) plotted against ΔT. (D-G) Centroid speed related traits (depicted in Figure 2A) plotted against ΔT. (H) Transition probabilities between various states: FP, forward to pause; FR, forward to reversal; FO, forward to omega; FF, forward to forward. (I) Phenotype barcodes for alphabetically ordered 49 strains at the indicated ΔT. Each behavioral feature is Z-score normalized as described in Methods. MRD, mean reversal duration; MPD, mean pause duration; MAXS, maximum speed after stimulus; TMAX, average time to reach maximum speed; ACCN, average acceleration after stimulus.