Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice

Table 4 Primers used for reporter-gene retrofitting

Gene	Primers (5′-3′)	Sequence^a
AMOTL1	Forward	CCGGCAGCCGTCTTCCCCAGCCGAGGGACTGAACTAGCCATGATCGCCTCatggcggatcccgtcgtttt
	Reverse	GTGGGAGACTCGGAGACGCCCTCCCGGCACCTCGAGTGGGGGCTGGTTACgaagttcctatactttctag
LCT	Forward	TTGCAGTTATAAAGTAAGGGTTCCACATACCTCCTAACAGTTCCTAGAAAatggtgagcaagggcgagga
	Reverse	TGTGTGATGAAGGTTGCCGAGGGGTCACCATCAGGTCAATGTGTACTCACgaagttcctatactttctag
MAOA	Forward	TTGCCGTCCCCACTCCTGTGCCTACGACCCAGGAGCGTGTCAGCCAAAGCatggcggatcccgtcgtttt
	Reverse	ACCCCTCACTGGCCAGGGTCCCCCAGGCCACCGCTACGGTCCACACTGACgaagttcctatactttctag
NGFR	Forward	CCGCAAAGCGGACCGAGCTGGAAGTCGAGCGCTGCCGCGGGAGGCGGGCGatggcggatcccgtcgtttt
	Reverse	GGAGTTCTGATCCCGGGAAAGGGAGCGGGCCCCCTCCGGCTAACACTCACgaagttcctatactttctag
NOV	Forward	TACAGCGAAGAAAGTCTCGTTTGGTAAAAGCGAGAGGGGAAAGCCTGAGCatggcggatcccgtcgtttt
	Reverse	ACCAAGGCGGGCAAAGTAACTTGGGGGCATCTTAAGGGTGTGCCACTTACgaagttcctatactttctag
NR2E1	Forward	GCCGGGACTCGGGCAGCGCCCACCAACCGCTCCGCCCCGGGACAGCCAGCatggcggatcccgtcgtttt
	Reverse	TCGCCCCAGGCTGCGCGCCTAGGCCCCACGGCGGCCCGAGAGGTACCCACgaagttcctatactttctag
NR2F2	Forward	CGCCGCCCGCAGCCAGGGGAGCAGGAAGTCCGGACGCAGCCCCCATAGATatggcggatcccgtcgtttt
	Reverse	CCAGGACCCCGGGACCCAGGACGAGGGAAGGAGAAATGAGAGGCCGATACgaagttcctatactttctag
PITX2	Forward	CCGCCGCTTCTTACAGCCTTCCTTCTCTTCTGTTTTGCAGATAACGGGGAatggcggatcccgtcgtttt
	Reverse	GTGGCGCGGCCTCCCGTCCGATGACCCGGGCAGGAGAAGGGGGTTCTTACgaagttcctatactttctag

^aCapital letters, sequence matching the mouse genome; lowercase letters, sequence matching the reporter gene (forward primer) or the kanamycin cassette (reverse primer).

ISSN: 1741-7007