Skip to main content

Table 4 Primers used for reporter-gene retrofitting

From: Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice

Gene Primers (5′-3′) Sequencea
AMOTL1 Forward CCGGCAGCCGTCTTCCCCAGCCGAGGGACTGAACTAGCCATGATCGCCTCatggcggatcccgtcgtttt
  Reverse GTGGGAGACTCGGAGACGCCCTCCCGGCACCTCGAGTGGGGGCTGGTTACgaagttcctatactttctag
LCT Forward TTGCAGTTATAAAGTAAGGGTTCCACATACCTCCTAACAGTTCCTAGAAAatggtgagcaagggcgagga
  Reverse TGTGTGATGAAGGTTGCCGAGGGGTCACCATCAGGTCAATGTGTACTCACgaagttcctatactttctag
MAOA Forward TTGCCGTCCCCACTCCTGTGCCTACGACCCAGGAGCGTGTCAGCCAAAGCatggcggatcccgtcgtttt
  Reverse ACCCCTCACTGGCCAGGGTCCCCCAGGCCACCGCTACGGTCCACACTGACgaagttcctatactttctag
NGFR Forward CCGCAAAGCGGACCGAGCTGGAAGTCGAGCGCTGCCGCGGGAGGCGGGCGatggcggatcccgtcgtttt
  Reverse GGAGTTCTGATCCCGGGAAAGGGAGCGGGCCCCCTCCGGCTAACACTCACgaagttcctatactttctag
NOV Forward TACAGCGAAGAAAGTCTCGTTTGGTAAAAGCGAGAGGGGAAAGCCTGAGCatggcggatcccgtcgtttt
  Reverse ACCAAGGCGGGCAAAGTAACTTGGGGGCATCTTAAGGGTGTGCCACTTACgaagttcctatactttctag
NR2E1 Forward GCCGGGACTCGGGCAGCGCCCACCAACCGCTCCGCCCCGGGACAGCCAGCatggcggatcccgtcgtttt
  Reverse TCGCCCCAGGCTGCGCGCCTAGGCCCCACGGCGGCCCGAGAGGTACCCACgaagttcctatactttctag
NR2F2 Forward CGCCGCCCGCAGCCAGGGGAGCAGGAAGTCCGGACGCAGCCCCCATAGATatggcggatcccgtcgtttt
  Reverse CCAGGACCCCGGGACCCAGGACGAGGGAAGGAGAAATGAGAGGCCGATACgaagttcctatactttctag
PITX2 Forward CCGCCGCTTCTTACAGCCTTCCTTCTCTTCTGTTTTGCAGATAACGGGGAatggcggatcccgtcgtttt
  Reverse GTGGCGCGGCCTCCCGTCCGATGACCCGGGCAGGAGAAGGGGGTTCTTACgaagttcctatactttctag
  1. aCapital letters, sequence matching the mouse genome; lowercase letters, sequence matching the reporter gene (forward primer) or the kanamycin cassette (reverse primer).