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Figure 7 | BMC Biology

Figure 7

From: IpsA, a novel LacI-type regulator, is required for inositol-derived lipid formation in Corynebacteria and Mycobacteria

Figure 7

Two-dimensional thin-layer chromatography (2D-TLC) analysis of [14C]-labeled polar lipids from Corynebacterium glutamicum cells grown in glucose (A-F) or myo -inositol (G-H). (A) and (G) C. glutamicum ATCC 13032, (B) and (H) ATCC 13032 ΔipsA, (C) ATCC 13032 ΔipsA pAN6-cg3323, (D) ATCC 13032 ΔipsA::pK18int-ipsA, (E) ATCC 13032 ΔipsA pAN6-DIP1969 and (F) ATCC 13032 ΔipsA pAN6-Rv3575. The cells were cultured in CGXII with either glucose (A-F) or myo-inositol (G, H). PI, phosphatidylinositol; TMCM, trehalose monocorynomycolate; GMCM, glucose monocorynomycolate; Ac1PIM2, monoacylated phosphatidyl myo-inositol dimannoside; GlcAGroAc2, 1,2-di-O-C16/C18:1-(α-d-glucopyranosyluronic acid)-(1→3)-glycerol (GL-A); ManGlcAGroAc2 1,2-di-O-C16/C18:1-(α-d-mannopyranosyl)-(1→4)-(α-d-glucopyranosyluronic acid)-(1→3)-glycerol (GL-X).

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