Figure 2

Functional target identification for top miRNA hits. (A and B) Predicted miRNA targets were compared to NF-κB signaling modulators identified in a previous genome-wide cDNA screen. (A) miRNAs that repressed NF-κB likely targeted activators of the pathway. (B) Conversely, miRNAs that activated NF-κB likely targeted repressors. (C) Scatterplot of the context scores of the predicted targets for each miRNA hit from the screen. y-axis is the negated context score (a more negative context score indicates a more confident prediction). Colors are alternated between black and grey for visual clarity. Solid black line is the median -context score for all miRNA-mRNA predictions (that is all 328 miRNAs and approximately 31,000 transcripts). Dashed line represents cutoff of 2 MAD from the median. MAD, median absolute deviations; miRNA microRNA; NF-κB, nuclear factor kappa-B.