Figure 6

miR-517a/c induce apoptosis in HEK293 and U-2 OS cells. (A) Microscope images of HEK293 and U-2 OS cells transfected with miR-517a/c. (B) Caspase3/7 activity was measured in HEK293 and U-2 OS cells transfected with miR-517a/c with the Caspase-Glo 3/7 luminescence assay. (C) Western blot analysis of whole cell protein extracts from HEK293 cells transfected with miR-517a/c. Cleavage of PARP is evident by the lower molecular weight fragment and is indicative of caspase3 activity. HSP90 was the loading control. (D) Microscope images of HEK293 cells transfected with TNIP1 siRNA. (E) Similar to (B) casapase 3/7 activity in HEK293 cells transfected with pooled siRNAs against TNIP1. (F) Western blot analysis of whole cell protein extracts transfected with pooled TNIP1 siRNAs. All values are means ± SD. n = 3. *P ≤0.05, **P ≤0.01. miRNAs, micro RNAs; PARP, poly(ADP-ribose) polymerase; siRNAs, small interfering RNAs; SD, standard deviation; TNIP1, tumor necrosis factor alpha-induced protein 3 interacting protein 1.