PmLox ectodermal domain of expression. (A, C, E) PmLox in situ hybridization developed with alkaline phosphatase methodology (A, E) and with fluorescent tyramide cy5 methodology coupled with DAPI staining (C, PmLox transcripts in green, nuclei in blue); B, D and F show acetylated tubulin immunostaining (in magenta) coupled with nuclear staining (in blue). A, B, E, Fvembryos and larvae are shown in frontal view; C and D larvae are shown in lateral view. Developmental stages are indicated in each panel. d, days; DAPI, 4',6-diamidino-2-phenylindole.