Figure 10
From: Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish
Endocrine cell differentiation is abolished in the double ascl1b and neurod1 morphants. Ventral views of WISH showing expression of insulin (A-B, I-J), somatostatin (C-D), ghrelin (E-F) and glucagon (G-H) in control (A,C,E,G,I) and Mo-ascl1b/Mo2-neurod1 (B,D,F,H,J) morphants at 38 hpf (A-H) or 17 hpf (I-J). The quantifications on the right side of the figure represent the number of positive cells per embryo for controls and double ascl1b/neurod1 morphants. Asterisks (***) indicate that the difference between cell number in controls and double ascl1b/neurod1 morphants is statistically significant by Student’s t-test (P <0.001). hpf, hours post fertilization; WISH, whole-mount in situ hybridization.