Endocrine cell differentiation is abolished in the double ascl1b and neurod1 morphants. Ventral views of WISH showing expression of insulin (A-B, I-J), somatostatin (C-D), ghrelin (E-F) and glucagon (G-H) in control (A,C,E,G,I) and Mo-ascl1b/Mo2-neurod1 (B,D,F,H,J) morphants at 38 hpf (A-H) or 17 hpf (I-J). The quantifications on the right side of the figure represent the number of positive cells per embryo for controls and double ascl1b/neurod1 morphants. Asterisks (***) indicate that the difference between cell number in controls and double ascl1b/neurod1 morphants is statistically significant by Student’s t-test (P <0.001). hpf, hours post fertilization; WISH, whole-mount in situ hybridization.