Growth and cell cycle analysis following RNAi of Tb11.NT.29. (A) Tb11.NT.29 RNAi in procyclics (PC). Growth for un-induced (-tet) and induced cells (+tet) shown in log scale. The data are based on three independent experiments, and average values with standard deviations are presented. (B) Analysis of the K/N configurations after induction of Tb11.NT.29 RNAi in procyclic cells. Cells were scored as having one kinetoplast and one nucleus (1K1N), two kinetoplasts and one nucleus (2K1N), two kinetoplasts and two nuclei (2K2N), one kinetoplast and no nucleus (1K0N), one kinetoplast and two nuclei (1K2N) and more than two nuclei (>2 N). The data are based on three independent experiments and at each time point 1,000 cells were counted. (C) Accumulation of cells with altered morphology after induction of Tb11.NT.29 RNAi in procyclic cells. (D) Example images of a normal cell and cells with altered morphology. Images for DNA content (Hoechst) and DIC are displayed. At each time point 500 cells were counted. DIC, differential interference contrast.