Hmga2 is expressed in the mouse embryonic lung at the distal airways. (A) Hmga2 is expressed during embryonic lung development. Hmga2 and Gapdh expression was monitored by quantitative RT-PCR in mouse embryonic lung at different days post coitum (E11.5 to E18.5) and in mouse lung at different days after birth (P1 to P28). Rel nor exp, relative expression normalized to Tuba1a. Error bars show the SEM (n = 4). (B) Hmga2 is expressed in the mouse embryonic lung at the distal airways. Hmga2 mRNA was detected in mouse embryonic lung at E12.5 and E14.5 by in situ hybridization using an Hmga2-specific antisense RNA probe. Sense probe, negative control. Scale bars, 200 μm. Square shows details at higher magnification. (C) HMGA2 localized in the cell nuclei of the embryonic lung. Fluorescence microscopy of embryonic lung sections (E14.5) after double immunostaining using HMGA2- and LMNB1-specific antibodies. Nuclear staining with DAPI (blue). Squares as in B. Scale bars, 40 μm.