Figure 1
From: Specific NuRD components are required for fin regeneration in zebrafish
One of the three Mi-2 orthologs, chd4a , is specifically induced in the blastema during fin regeneration. (A) Quantitative real-time-PCR analysis of chd4a, chd4b, and chd3 mRNA in regenerating adult caudal fins at 1 day post-amputation (dpa) and 3 dpa relative to control fins at 0 hours post-amputation (hpa). Error bars represent the SEM. *P < 0.001 (B-D) In situ hybridization with chd4a (B), chd4b (C), and chd3 (D) mRNA antisense probes on cryosections of regenerating adult caudal fins at 3 dpa. Dashed lines indicate the amputation plane. (E-G) Whole-mount in situ hybridization with chd4a (E), chd4b (F), and chd3 (G) antisense probes in 1 dpa larval tails. Scale bar: 100 μm.