Figure 2
From: Specific NuRD components are required for fin regeneration in zebrafish
Specific NuRD component orthologs are expressed in the blastema of regenerating fins. (A) Quantitative real-time-PCR analyses of hdac1, mta1, mta2, mta3, rbb4, rbb4l, mbd3a, mbd3b, and mbd2 mRNA in regenerating adult caudal fins at 1 and 3 dpa relative to control fins at 0 hpa. Error bars represent the SEM. *P < 0.001 (B-D) In situ hybridization with hdac1 (B), rbb4 (C), and mta2 (D), antisense probes on cryosections of regenerating adult caudal fins at 3 dpa. Dashed lines indicate the amputation plane. (E-G) Whole-mount in situ hybridization with hdac1 (E), rbb4 (F) and mta2 (G) antisense probes in 1 dpa larval tails. Scale bar: 100 μm.