No display of male-typical behaviors in cGαo-/-females. (A-D) B6 and cGαo-/- females display little or no mounting behavior toward male, female, or castrated male intruders (analysis of variance (ANOVA): F1,56 = 3.339 to 6.286, P = 0.06 to 0.88). B6 and cGαo-/- male mice show similar mounting behaviors toward female intruders (t-test: P = 0.49 to 0.96). (E,F) Aggression levels toward males remain low in both B6 and cGαo-/- female mice (ANOVA: F1,56 = 2.182, P = 0.15 (attack duration)). (G) There are similar intruder investigation times for both B6 and cGαo-/- female mice (ANOVA: F1,56 = 0.653, P = 0.42).