No impact of the conditional Gαo deletion on Gαo expression in reproductive central nervous system nuclei. (A-L) Representative olfactory marker protein (OMP) immunohistochemistry in the paraventricular nucleus and medial preoptic area of the hypothalamus as well as the medial amygdala in B6 and cGαo-/- female mice. Schematics on the left indicate the studied brain areas (red). Double immunostaining for OMP (arrowheads) and Gαo in cGαo-/-(B,F,J) and B6 females (D,H , L) in the same brain areas reveal no overlap between both signals. Scale bar, 100 μm (A-K) and 50 μm (J,L).