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Table 4 Effect of combining the mutation in the exonuclease domain of the Pol δ gene with the active site mutation in Pol ε genes on mutation rates in mismatch repair-proficient derivatives of 8C-YUNI101 strain

From: Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations

Relevant genotype Mutation rates for different markers
  Forward mutation Reversion
  Canr × (10-7) Base substitutions Ura+ (× 10-8) Frameshifts (+1) His+ (× 10-8)
wild-type 2.9 1.5 1.5
  1.6–4.9 1.1–2.3 1.2–2.1
+/pol3-01* 28 n/a 33
  22–28   31–39
+/pol3-01*pol2-Y831A 31 n/a 27
  25–39   24–34
pol3-01 190 19 110
  140–260 6–35 80–160
pol3-01 pol2-Y831A 130 14 34
  90–210 13–28 18–43
  1. n/a, non applicable, since these strains are Ura+. *Haploids with duplication of the POL3 gene.
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