Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations

Table 4 Effect of combining the mutation in the exonuclease domain of the Pol δ gene with the active site mutation in Pol ε genes on mutation rates in mismatch repair-proficient derivatives of 8C-YUNI101 strain

Relevant genotype	Mutation rates for different markers
	Forward mutation	Reversion
	Can^r × (10^-7)	Base substitutions Ura⁺ (× 10^-8)	Frameshifts (+1) His⁺ (× 10^-8)
wild-type	2.9	1.5	1.5
	1.6–4.9	1.1–2.3	1.2–2.1
+/pol3-01*	28	n/a	33
	22–28		31–39
+/pol3-01*pol2-Y831A	31	n/a	27
	25–39		24–34
pol3-01	190	19	110
	140–260	6–35	80–160
pol3-01 pol2-Y831A	130	14	34
	90–210	13–28	18–43

n/a, non applicable, since these strains are Ura⁺. *Haploids with duplication of the POL3 gene.

ISSN: 1741-7007