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Table 1 Sequences of individual clones from the randomized U5-IR stem librarya.

From: Selection of functional mutations in the U5-IR stem and loop regions of the Rous sarcoma virus genome

Clone ID

83

84

85

86

87–95b

96

97

98

99

Wild Type

U

G

A

A

GCAGAAGGC

U

U

C

A

IRS-1

U

G

A

G

 

U

U

C

A

IRS-2

U

A

A

G

 

U

U

U

A

IRS-3

C

A

A

U

 

G

C

A

U

IRS-4

G

A

G

U

 

C

A

U

U

IRS-5

U

G

C

C

 

A

G

G

A

IRS-6

U

G

G

G

 

C

G

C

A

IRS-7

U

G

G

A

 

U

U

C

A

IRS-8

G

G

A

U

 

U

G

C

A

IRS-9

U

A

U

G

 

C

A

U

A

IRS-10

U

A

A

U

 

A

C

A

U

IRS-11

C

G

A

U

 

U

G

C

A

IRS-12

U

G

A

U

 

A

U

C

A

IRS-13

A

C

C

G

 

G

A

C

U

IRS-14

U

U

G

G

 

A

C

C

A

IRS-15

A

G

U

G

 

U

A

U

U

IRS-16

G

A

U

A

 

U

U

C

A

IRS-17

U

G

U

G

 

G

G

C

A

IRS-18

A

A

A

C

 

A

U

G

A

IRS-19

C

G

A

U

 

U

G

C

A

  1. aIndividual clones were recovered after two rounds of infection and sequenced as described under "Methods". bRNA nucleotide positions 87–95 were not randomized in this library and were included to indicate the nucleotides between the two randomized regions.
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BMC Biology

ISSN: 1741-7007

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