Skip to main content

Table 1 Rescue of gastrulation in SpRunt-1 morphants by Ac-DEVD-CHO. Embryos injected with SpRunt-1 MASO were divided into two groups, one of which was treated with Ac-DEVD-CHO as described in the Methods. At 48 h the embryos were observed under a stereomicroscope and scored as gastrulation defective (with a typical morphant phenotype similar to that depicted in Figure 2A), rescued gastrulation (with guts but no skeletons, a phenotype similar to that depicted in Figure 2D), or normal (indistinguishable from uninjected control embryos with skeletons). The latter group probably represents embryos that were under injected; note that this particular experiment had an unusually high proportion of such embryos (which more typically are in the range of 10–15%).

From: Runx-dependent expression of PKC is critical for cell survival in the sea urchin embryo

Treatment Gastrulation defective Rescued Gastrulation Normal
SpRunt-1 MASO alone (n = 259) 162 (63%) - 97 (37%)
SpRunt-1 MASO + Ac-DEVD-CHO (n = 158) 59 (37%) 55 (35%) 44 (28%)