Figure 3

Wnt -5 has a specific role in islet formation. (A) Double in situ hybridization with pdx-1 and wnt-5, 10 som stage, dorsal view, the anterior is to the left. Arrow, pdx-1 expression, bracket, wnt-5 expression. (B-H) In situ hybridization with insulin at 24 hpf. (B) wild-type, (C) WNT-8 morphant embryos, (D) WNT-11 morphant embryos, (E) WNT-5 morphant embryos, (F)wnt-5 mismatch MO-injected embryos, (G) wnt-5 RNA injected embryo, (H) wnt-5 MO and wnt-5 RNA co-injected embryo. Notice the compact islet in this embryo that displays an undulated notochord. (I) Percentage of embryos with scattered insulin expression resulting from injection of wnt-5 MO reduced significantly from 60% to 10% when wnt-5 RNA was co-injected with wnt-5 MO. (J-L) Morphology at 24 hpf, (J) wild-type, (K) wnt-5 insertional mutant, (L) wnt-5 translation-blocking MO-injected embryos. Notice that wnt-5 MO injected embryos have more severe morphological phenotype than wnt-5 insertional mutant embryos. (M) RT-PCR analysis of wnt-5 transcript in wnt-5 exon-intron MO injected embryos. Injection of wnt-5 exon-intron MO results in severely shortened wnt-5 transcript. L:ladder, 1:EF-1α control, 2:wnt-5.