Skip to main content

Advertisement

Table 1 Wnt-5 heterozygous embryos are more sensitive to wnt-5 MO injection in pancreatic islet formation.

From: Wnt5 signaling in vertebrate pancreas development

  WT WT X wnt-5/WT
  trans-block wnt-5 MO exon-intron wnt-5 MO trans-block wnt-5 MO exon-intron wnt-5 MO
% of embryos with abnormal insulin expression 5 ± 2% 0% 46 ± 5% 52 ± 3%
total # of embryos N = 124 N = 113 N = 53 N = 57
  1. 2 ng of either wnt-5 translation-blocking or exon-intron junction targeting morpholinos were injected into either wild-type embryos or embryos generated from an outcross of a wnt-5 mutant carrier adult. In wild-type embryos, injection of either wnt-5 MO resulted in less than 5% of embryos with scattered insulin expression. However, injection of either wnt-5 MO resulted in about 50% of embryos with scattered insulin expression in embryos obtained from the wnt-5 mutant carrier outcross.