Figure 3

Muscle fibres of the ovary. A,B. Confocal fluorescent microscopy showing the muscle fibres of the ovariolar epithelial sheath, ES, and the ovarian peritoneal sheath, PS. The peritoneal sheath fibres overlie those of the epithelial sheath. In A, myosin (green) is Mhc-weeP26-GFP. In B, FITC-labelled anti-myosin antibody is green, with F-actin (magenta) detected with rhodamine-labelled phalloidin. Note that the ovariolar muscle fibres all circle the ovariole, whereas the peritoneal sheath muscle fibres form a meshwork across the ovarial surface. C, D. Electron micrographs of the peritoneal sheath (PS) and epithelial sheath (ES) respectively, showing longitudinal sections of sarcomeres with interdigitating thick and thin filaments (white arrow) and perforated Z-discs (black arrow). EC: egg chamber. Scalebars: A: 50 μm; B: 10 μm, C:200 nm, D:500 nm.