Figure 4

Time-lapse imaging of the wild-type and Δhinge Myo1 ring contraction. Typical examples of Myo1-GFP-(myc)6 (RLY1331) and Myo1Δhinge-GFP-(myc)6 (RLY2139) CR contraction as shown as a montage with images one minute apart (A) and as kymographs (B). Each horizontal line of the kymograph represents a time point of the image series with images taken at 30 s intervals, derived from a line drawn across the GFP signal at the bud neck (shown in the schematic below B). These lines are then stacked next to each other in chronological order to generate the kymograph. In A, scale bars denote 5 μm.