Figure 2 From: Expression of mammalian GPCRs in C. elegansgenerates novel behavioural responses to human ligands Heterologously expressed GPCRs are integrated into an endogenous signalling pathway in C. elegans. Mutation of the TRPV channel subunit osm-9 and the Gα subunit gpa-3 fully abolished avoidance of (A) 25 μM SST-28 in Sstr2 transgenic animals and (B) 25 μM MIP-1α in CCR5 transgenic animals. Mutation of Gα subunit odr-3 did not affect these responses. (C) In contrast, mutation of either gpa-3 or odr-3 did not have a significant effect on avoidance response in sra6::CCR5 transgenic animals. Each data point represents an average of at least 3 independent assays for wild type and each respective strain. Error bars denote the standard error of the mean. Asterisks indicate a statistically significant difference between osm-9 or gpa-3 mutant animals and wild type animals carrying the Sstr2 or CCR5 transgenes (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005).Back to article page