acr-2::YFP and acr-5::CFP expression overlaps in the ventral cord, but not in the tail of the male. Lateral views with anterior oriented left, and dorsal oriented upwards (A–L) expression of acr-5::CFP and acr-2::YFP in the male tail at three focal planes: (A–D), (E–H) and (I–L). Nomarski (A, E, I), acr-5::CFP fluorescence (B, F, J), acr-2::YFP fluorescence (C, G, K) and merged (D, H, L) images. (M–P) overlap of acr-2::YFP and acr-5::CFP in the ventral cord. Nomarski (M), acr-5::CFP fluorescence (N), acr-2::YFP fluorescence (O) and merged (P) images. Animals shown are pha-1(e2123ts) young adult males transformed with the extrachromosomal array pha-1+ acr-2::YFP and carrying the extrachromosomal array acr-5::CFP that was crossed into the strain.