Figure 4

Comparison of SpCBFβ and SpRunt-1 morphant phenotypes. DIC images of (A) 24 h and (B) 72 h control MASO injected embryos; arrow in (B) points to skeletal rod (spicule); (C) 24 h and (D) 72 h SpRunt-1 MASO injected embryos; and (E) 24 h and (F) 72 h SpCBFβ MASO injected embryos; black arrow in (F) points to tripartite gut, and white arrowhead points to a "ventrolateral" cluster of skeletogenic mesenchyme cells. Scale bars = 20 μm; embryos in (A), (C), (D) and (E) are to the same scale. (G) Immunoblot of total blastula-stage embryo extract showing that the SpCBFβ MASO is effective in depleting endogenous SpCBFβ (arrow).