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Table 1 EST library IDs categorized by the description of developmental stages. Each categorized mouse developmental stage contained about 22,300 (409–71,504) EST counts on average, which corresponded to 2,900 (194–5,923) non-redundant ENSEMBL gene entries on average. Developmental status was based on Manipulating the Mouse Embryo) [27] and The Mouse [28].

From: The vertebrate phylotypic stage and an early bilaterian-related stage in mouse embryogenesis defined by genomic information

Stage

Developmental status

dbEST library ID

1

Unfertilized egg

16178, 16177, 10029, 14142, 1389, 867

2

Fertilized egg, 1 cell

12233, 2589, 12241, 1119, 17903

3

2 Cell

862, 1382, 5391

4

4 Cell

12243, 15709, 1524, 7328

5

8 Cell

9845, 15708, 1381

6

Morula

12244, 1532, 18029

7

Blastocyst, E4.0

12245, 10026, 1310, 1021, 875, 850, 18027, 12246, 18021

8

Trophoblast, ES cell

10033, 10023, 14556, 915, 10025, 10024, 14558

9

Formation of egg cylinder (E5.0)

12231, 18022

10

Proamniotic cavity in primitive ectoderm (E6.0)

2565

11

Advanced endometrial reaction, gastrulation starts, embryonic axis determined (E6.5)

13851, 10561

12

Primitive streak, amnion forming (E7.0)

5422, 547, 2381, 375, 2790, 12316

13

Neural plate, presomite stage (E7.5)

10036, 3777, 10562, 844

14

Neural folds, otic placode, 1–7 somites, hindgut pocket, 1st pharyngeal arch (E8.0)

2566, 1913

15

Neural folds close, 8–12 somites, 1st and 2nd pharyngeal arch, turning of embryo (E8.5)

10039, 538, 4042

16

Formation and closure of anterior neuropore, olfactory placode, 13–20 somites (E9.0)

5432, 7256

17

Forelimb bud, 21–29 somites (E9.5)

5392, 13850

18

Hindlimb and talbud, 30–34 somites (E10)

1884, 2517, 16788

19

Deep lens pit, 35–39 somites (E10.5)

1891, 15706, 528

20

Closure of lens vesicle, 40–44 somites (E11.0)

1885, 4142, 12312, 2557, 2578, 9944, 18018

21

Forefoot plate, lens vesicle detached (E11.5)

15707, 845

22

Hind foot plate, earliest sign of fingers (E12.0)

5319, 9846, 5464, 5423, 2514

23

Anterior footplate indented, (E13.0)

12258, 2521, 2554, 2521, 5469, 2555

24

Skin layers, chondrification of ribs (E13.5)

532, 15702

25

Fingers separate distally (E14.0)

12259, 2525

26

Fingers & toes separate (E15.0)

17914, 2596, 12265, 2522, 17951, 2594

27

Rib ossification (E15.5)

529, 12979

28

Reposition of umbilical hernia (E16.0)

12260, 2523, 18085, 12247

29

Wrinkled skin, umbilical hernia gone (E17.0)

12261, 18071, 12250, 12248, 12251, 2516, 1907

30

Auditory ossicles (E17.5)

12267, 18087

31

Iris and ciliary body (E18.0)

12266, 1770, 12313