Analysis of FIGLER expression in hematopoietic cells and cell lines. (A) RT-PCR analysis of human FIGLER 1–5 mRNA transcripts. Panel 1, primary bone marrow B lineage cells: CD34+CD19-, hemopoietic stem cells and other early hemopoietic cells; CD34+CD19+, pro-B cells; CD34-CD19+IgM-, pre-B cells; and CD34-CD19+IgM+ B cells. Panel 2, bone marrow-derived stromal cell lines. Panel 3, primary thymus and bone marrow non-B lineage cells. (B) RT-PCR analysis of FIGLER 1, 2 and 9 mRNA transcripts in human pro-B (REH, Nalm16), pre-B (697, 207, OBS), B (Ramos, Daudi, Raji), T (Jurkat), myeloid (THP-1, U937, HL-60, KG-1), and erythoid (K562) cell lines. FIGLER 9 (Pal), a retina specific protein, served as a negative control and β-actin served as a loading control for all these analyses. PCR product identity was confirmed by sequencing as described in the Methods section.