Figure 2

Characterization of androgen-responsive non-coding intronic RNAs. Selected intronic transcripts extended by RACE-PCR (A-D, red boxes) or gene-specific PCR (E, yellow box) are shown. Extended unspliced antisense intronic transcript fragments were mapped to the genomic sequence (A-E, gray lines) relative to the respective spliced protein-coding transcript expressed in the opposite strand (A-E, green arrows). Double-stranded cDNA clones spotted on the microarrays are represented as blue boxes (A-E). A previously described antisense unspliced intronic transcript mapping to GAS6 locus is shown (E, black arrow). Chromosome coordinates and the length of extended intronic transcript fragments are indicated. (F) Strand-specific multi-tissue northern blot using probes complementary to the antisense intronic transcript mapped in the GAS6 locus.