Figure 7

Live cell imaging of mWasabi fusion vectors. (A)-(J) N-terminal fusion constructs; for each fusion protein the linker amino acid length is indicated after the name of the targeted organelle or fusion protein: (A) mWasabi-α-actinin-19 (human non-muscle); (B) mWasabi-mitochondria-7 (human cytochrome C oxidase subunit VIII); (C) mWasabi-Cx26-7 (rat β-2 connexin-26); (D) mWasabi-keratin-17 (human cytokeratin 18); (E) mWasabi-paxillin-22 (chicken); (F) mWasabi-EB3-7 (human microtubule-associated protein; RP/EB family); (G) mWasabi-golgi-7 (N-terminal 81 amino acids of human β-1,4-galactosyltransferase); (H) mWasabi-vimentin-7 (human); (I)mWasabi-H2B-6 (human); (J) mWasabi-zyxin-7 (human). (K)-(T) C-terminal fusion constructs: (K) mWasabi-lamin B1-10 (human); (L) mWasabi-β-actin-7; (M) mWasabi-β-tubulin-6 (human); (N) mWasabi-clathrin light chain-15 (human); (O) mWasabi-vinculin-23 (human); (P) mWasabi-farnesyl-5 (20-amino acid farnesylation signal from c-Ha-Ras); (Q) mWasabi-focal adhesion kinase-5 (chicken protein tyrosine kinase 2); (R) mWasabi-peroxisomes-2 (peroximal targeting signal 1; PTS1); (S) mWasabi-endosomes-15 (human RhoB GTPase with an N-terminal c-Myc epitope tag); (T) mWasabi-annexin (A4)-15 (human). The cell line used for expressing mWasabi fusion vectors was gray fox lung fibroblast cells (FoLu) in panels (E) and (F) and human cervical adenocarcinoma cells (HeLa) in the remaining panels. Scale bars represent 10 μm.