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Table 1 Properties of hue-shifted mTFP1 variants

From: Hue-shifted monomeric variants of Clavulariacyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging

  Excitation maximum (nm) Emission maximum (nm) ε(mM-1 cm-1) Φ Brightnessa (mM-1 cm-1) pKa Photostabilityb
mTFP1-Y67W 424/440c 461/482c 13 0.02 0.3 NDd ND
mTFP1-Y67H 369e NAf 7 NA NA ND NA
G1 487 503 43 0.60 26 ND ND
G2 487 503 60 0.65 39 ND 65
G3 498 515 70 0.70 49 ND 5.5
mWasabi 493 509 70 0.80 56 6.5 93
mTFP1g 462 492 64 0.85 54 4.3 110
EGFPh 488 507 56 0.60 34 6.0 174
  1. aProduct of extinction coefficient and quantum yield.
  2. bTime in seconds to bleach from an initial emission rate of 1000 to 500 photons s-1 molecule-1.
  3. cValues for both 'humps' are provided.
  4. dND, not determined.
  5. eThis value is the absorption maximum. No significant fluorescence was detected for mTFP1-Y67H.
  6. fNA, not applicable.
  7. gValues from [19].
  8. hValues from [7].