Hue-shifted monomeric variants of Clavulariacyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging

Table 1 Properties of hue-shifted mTFP1 variants

	Excitation maximum (nm)	Emission maximum (nm)	ε(mM^-1 cm^-1)	Φ	Brightness^a (mM^-1 cm^-1)	pKa	Photostability^b
mTFP1-Y67W	424/440^c	461/482^c	13	0.02	0.3	ND^d	ND
mTFP1-Y67H	369^e	NA^f	7	NA	NA	ND	NA
G1	487	503	43	0.60	26	ND	ND
G2	487	503	60	0.65	39	ND	65
G3	498	515	70	0.70	49	ND	5.5
mWasabi	493	509	70	0.80	56	6.5	93
mTFP1^g	462	492	64	0.85	54	4.3	110
EGFP^h	488	507	56	0.60	34	6.0	174

^aProduct of extinction coefficient and quantum yield.
^bTime in seconds to bleach from an initial emission rate of 1000 to 500 photons s^-1 molecule^-1.
^cValues for both 'humps' are provided.
^dND, not determined.
^eThis value is the absorption maximum. No significant fluorescence was detected for mTFP1-Y67H.
^fNA, not applicable.
^gValues from [19].
^hValues from [7].

ISSN: 1741-7007