Figure 3

Analysis of the SPG4 minimal promoter. (a) Sequence comparison using mVISTA between human and mouse genomic regions upstream of the first ATG of SPG4 demonstrates extensive sequence conservation. (b) Schematic representation of the constructs used. Different genomic sequences were cloned upstream of the firefly luciferase reporter gene. The arrow indicates the transcriptional start sites of the reference SPG4 sequence. The position of a putative CAAT box is shown. (c) HeLa, SH-SY-5Y and HEK293 cells were cotransfected with the indicated constructs and with a CMV-Renilla luciferase plasmid. Cell lysates were prepared 24 hours post-transfection and the activity of the firefly luciferase was normalized to that of Renilla luciferase. For each construct at least three independent experiments were performed using different DNA preparations. Error bars represent standard error of the mean.