Expression of all predicted α- and β-like globin genes including GBY in an adult platypus. For each of the platypus predicted genes, expression was investigated by reverse transcriptase polymerase chain reaction in adult liver, kidney, spleen, testis, brain and lung. Primers for each gene were designed between two exons so that it would result in a product distinguishable from genomic contamination of cDNA. The negative control (last lane) contained no cDNA. All genes were expressed in one or more tissues, indicating that they are transcriptionally active and might be functional.