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Table 1 General characteristics of Sitophilus zeamais ESTs from suppressive subtractive hybridization between E. coli-infected and naive larvae

From: Identification of the Weevil immune genes and their expression in the bacteriome tissue

Total number of cDNA reads 485
Total number of cDNAs analyzed 475
Average ESTs length (bp) 373
cDNA assembly a result  
   Number of ESTs in contigs 264
   Number of contigs 62
   Number of singletons 211
   Number of consensus 273
Redundancy b 55%
   Number of contigs containing  
2 to 4 ESTs 49
5 to 10 ESTs 8
> 10 ESTs 5
Functional classification  
   No Uniprot c hits 17%
   No GO assignment 37%
   GO Biological Process (Level 2) Number of contigs
Reproduction 2
Immune system process 6
Metabolic process 82
Cellular process 71
Viral reproduction 2
Reproductive process 2
Biological adhesion 3
Multicellular organismal process 4
Developmental process 7
Growth 1
Response to stimulus 13
Localization 26
Establishment of localization 26
Multi-organism process 1
Biological regulation 11
  1. a. TGICL parameters: ESTs with 94% identity over at least 30 base pairs and a maximum length of unmatched overhangs of 30 nucleotides were clustered together.
  2. b. Redundancy = number of ESTs in contigs/Total number of ESTs.
  3. c. UniProt Rel. 10 (SWISS-PROT 52 + TrEMBL 35) April 10, 2007, cut-off E-value was 10.
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