Identification of the Weevil immune genes and their expression in the bacteriome tissue

Table 1 General characteristics of Sitophilus zeamais ESTs from suppressive subtractive hybridization between E. coli-infected and naive larvae

Total number of cDNA reads	485
Total number of cDNAs analyzed	475
Average ESTs length (bp)	373
cDNA assembly ^a result
Number of ESTs in contigs	264
Number of contigs	62
Number of singletons	211
Number of consensus	273
Redundancy ^b	55%
Number of contigs containing
2 to 4 ESTs	49
5 to 10 ESTs	8
> 10 ESTs	5
Functional classification
No Uniprot ^c hits	17%
No GO assignment	37%
GO Biological Process (Level 2)	Number of contigs
Reproduction	2
Immune system process	6
Metabolic process	82
Cellular process	71
Viral reproduction	2
Reproductive process	2
Biological adhesion	3
Multicellular organismal process	4
Developmental process	7
Growth	1
Response to stimulus	13
Localization	26
Establishment of localization	26
Multi-organism process	1
Biological regulation	11

^a. TGICL parameters: ESTs with 94% identity over at least 30 base pairs and a maximum length of unmatched overhangs of 30 nucleotides were clustered together.
^b. Redundancy = number of ESTs in contigs/Total number of ESTs.
^c. UniProt Rel. 10 (SWISS-PROT 52 + TrEMBL 35) April 10, 2007, cut-off E-value was 10.

ISSN: 1741-7007