Figure 9
Pulsed field gel electrophoresis of the symbiont genomic DNA prepared from dissected midgut crypts of the acanthosomatid stinkbugs. (A) Elasmostethus humeralis. (B) Elasmostethus nubilus. (C) Sastragala esakii. Lane M1, size marker (Saccharomyces cerevisiae chromosomes); lane M2, size marker (lambda ladder); lane S1, I-CeuI digestion for E. humeralis; lane S2, I-CeuI digestion for E. nubilus; lane S3, AscI digestion for E. nubilus; lane S4,ApaI digestion for S. esakii; lane S5, SmaI digestion for S. esakii. Sizes of genomic DNA fragments are depicted on the gel images. Estimated genome sizes are shown under the gel images.