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Table 1 Effect of surface sterilization of eggs on symbiont acquisition in Elasmostethus humeralis.

From: Host-symbiont co-speciation and reductive genome evolution in gut symbiotic bacteria of acanthosomatid stinkbugs

  Symbiont detection (no. positive/total)
Egg mass number Control Surface sterilization a,b
1 6/6 0/6
2 8/8 0/12
3 15/16 0/18
4 13/13 0/14
5 7/8 0/9
6 6/7 0/7
7 7/7 0/6
8 20/20 0/18
Total 82/85 0/90
  1. aEggs were treated with 70% ethanol for 10 min and 4% formaldehyde containing 0.1% Triton X-100 for 30 min, and rinsed two times with 70% ethanol.
  2. bAll samples showed no amplification after 35 cycles of PCR.