|
Symbiont detection (no. positive/total)
|
---|
Egg mass number
|
Control
|
Surface sterilization a,b
|
---|
1
|
6/6
|
0/6
|
2
|
8/8
|
0/12
|
3
|
15/16
|
0/18
|
4
|
13/13
|
0/14
|
5
|
7/8
|
0/9
|
6
|
6/7
|
0/7
|
7
|
7/7
|
0/6
|
8
|
20/20
|
0/18
|
Total
|
82/85
|
0/90
|
-
aEggs were treated with 70% ethanol for 10 min and 4% formaldehyde containing 0.1% Triton X-100 for 30 min, and rinsed two times with 70% ethanol.
-
bAll samples showed no amplification after 35 cycles of PCR.