Microarray analysis validation for GN-11 neurons. Microarray data (Table 1 and Additional file 1) were validated by quantitative real-time polymerase chain reaction (qRT-PCR) performed in triplicate for 13 genes on two different RNA preparations from control pSUPER-empty or AP-2α low-expressing (α4-c) clones. The 18S or GAPDH housekeeping gene was used as an internal control to normalize the data. Microarray analysis and qRT-PCR fold changes are shown for each validated gene as average values. Bars represent ± standard deviations.