The binding of recombinant AprA to cells. The indicated cells (WT is wild-type) were incubated with different concentration of recombinant AprA (rAprA). After 10 min, bound rAprA was quantitated by Western blots (staining for the Myc tag), using known amounts of rAprA as standards. Values are mean ± s.e.m. (n = 3). The lines are curve fits to a one-site binding model with no cooperative binding with the exception of the fit to the binding to gα5
- cells, where the line is a fit to a one-site binding model with a Hill coefficient of 2.9.