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Table 2 The effect of G proteins on the mass and protein content of cells

From: A Dictyostelium chalone uses G proteins to regulate proliferation

 

Per 107 cells

Percent of cells with n nuclei

Per 107 nuclei

Cell type

Mass, mg

Protein, mg

1

2

3 +

Nuclei/100 cells

Mass, mg

Protein, mg

Wild-type

10.8 ± 0.6

0.32 ± 0.01

76 ± 3

21 ± 2

3 ± 1

129 ± 5

8.4 ± 0.6

0.25 ± 0.01

gα2 -

10.7 ± 0.3

0.33 ± 0.01

72 ± 3

22 ± 2

6 ± 2

134 ± 6

8.0 ± 0.5

0.25 ± 0.02

gα5 -

12.1 ± 0.5

0.25 ± 0.01

59 ± 6

31 ± 4

11 ± 3

154 ± 8

7.9 ± 0.6

0.16 ± 0.01*

gα7 -

8.8 ± 0.3

0.28 ± 0.01

68 ± 6

25 ± 4

6 ± 2

140 ± 9

6.3 ± 0.5

0.20 ± 0.01

gα8 -

15.5 ± 0.1**

0.40 ± 0.02

56 ± 5*

30 ± 4

14 ± 2**

166 ± 8*

9.3 ± 0.5

0.24 ± 0.02

gα9 -

16.3 ± 1.5**

0.42 ± 0.01*

78 ± 4

20 ± 3

2 ± 1

124 ± 4

13.2 ± 1.3**

0.34 ± 0.02*

-

15.8 ± 1.3**

0.47 ± 0.05**

52 ± 5*

32 ± 2

17 ± 2**

180 ± 9**

8.8 ± 0.9

0.26 ± 0.03

  1. The mass and protein content of cells was measured as described in the Materials and Methods, and the percent of cells with 1, 2, 3, or more nuclei was measured by counts of DAPI-stained cells. After calculating the average number of nuclei per 107 cells, the mass and protein per 107 nuclei was calculated. All values are means ± SEM's from three or more independent assays. A * after a value indicates that the value was significantly different from the wild-type value with p < 0.05; ** indicates p < 0.01(1-way ANOVA, Dunnett's test).