TBP2 is associated with active promoters in oocytes. (A) Chromatin immunoprecipitation (ChIP) assay on germinal vesical (GV)-injected promoters and endogenous 5S rRNA promoters using TBP2 and pol-II antibodies. Enrichment is the signal relative to background which in this case is the ChIP recovery from a non-promoter region (intron construct). (B) ChIP assay on GV-injected pol-II promoters and endogenous 5S rRNA promoter from oocytes injected with HA-TBP2 mRNA. Enrichment is the signal relative to background which in this case is the ChIP signal from oocytes not expressing HA-TBP2 but injected with the same promoter construct. Error bars in (A) and (B) represent the standard error of the mean from three independent experiments.