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Table 1 Characteristic kinetic constants for the hydrolysis of ATCh and BTCh by DmAChE, HuAChE and HuBChE

From: Evidence for inhibition of cholinesterases in insect and mammalian nervous systems by the insect repellent deet

  DmAChE (ATCh) HuAChE (ATCh) HuBChE (BTCh)
Substrate    
k 3 [s-1] 395.4 13471 ± 2171 1082 ± 44
K ip [mM] 0.17 5.43 ± 1.0 11.3 ± 0.5
K L 4.17 0.0181 ± 0.0054 0.0019 ± 0.0008
K LL 179 9.23 ± 6.58 12.3 ± 5.4
k 2 [s-1] 53465 14375 ± 2182 420 ± 20
A 3.44 1.64 ± 0.36 1.32 ± 0.07
B 0.049 0.11 ± 0.03 39.6 ± 13.5
K s = K p *K L [mM] 0.73 0.098 0.021
K ss = K p *K LL [mM] 31.1 50.1 139
DEET    
K ip [mM] 1.02 ± 0.03 8.39 ± 6.97 0.37 ± 0.05
K iL   0.083 ± 0.078 1.54 ± 0.45
K iLL   0.56 ± -0.41 29 ± 5.4
c   1.49 ± 0.99 1.32
d   0.19 ± 0.18 0.52 ± 0.09
K is = K ip *K iL [mM]   0.69 0.57
K iss = K ip *K iLL [mM]   4.67 1.08
  1. ATCh = acetylthiocholine; BTCh = butyrylthiocholine; DmAChE = Acetylcholinesterase from D. melanogaster; HuAChE = Acetylcholinesterase from human; HuBChE = Butyrylcholinesterase from human.