Figure 6

Regulation of the hydrophobin genes hfb1 and hfb2 by GNA1. Mycelia were grown in Mandels-Andreotti medium supplemented with 1% (w/v) microcrystalline cellulose as carbon source in constant light (1,800 lux, 25 μmol photons m^-2 s^-1), indicated by LL, or constant darkness (DD) and harvested after 72 and 96 h. For northern blotting, 20 μg of total RNA were loaded per lane and [α³²P]-radiolabeled PCR fragments of hfb1, hfb2 and 18S rRNA were used as probes.