Sequence alignment and hybridisation network showing the symbiont of Coccidula rufa to be a recombinant. (a) Alignment of concatenated genes atpA, coxA, gltA, 16S showing just polymorphic sites. Nucleotides that are identical to the C. rufa sequence are shown as a dot. The (s)C. rufa sequence of atpA and coxA (shaded) are most similar to (s)Elateridae in the bellii group, while the gltA and 16S sequences (unshaded) are most similar to (s)Pediobius rotundus in the transitional group. (b) A hybridisation network of the concatenated sequences of atpA, coxA, gltA and 16S. A neighbour-net split network was generated and splits were then filtered by weight to include only the (s)C. rufa split. A hybridisation network was then performed on the split network to provide an explicit example of descent from the two different groups.