Dissection of the sensory cue that causes male's sexual discrimination against ci. (a) Males' discrimination against xanthophore-less females. Males of various strains (n = 12) were offered three female combinations of b
g8and either of HNI, ci, or ci-lf (n = 18; 6 for each strain). Note the biased preferences in the choices of b
g8-ci and b
g8-ci-lf. The one-way repeated measures ANOVA and the post-hoc LSD (but not Bonferroni) test, however, detected significant difference even between these choices (F
2,22 = 16.730, P < 0.001; post-hoc LSD, P = 0.022; post-hoc Bonferroni, P = 0.067). Hence, the increased leucophores may additively contribute to the unattractiveness of ci. (b) Male's sexual discrimination against ci recreated under non-free-swimming conditions. Males of b
g8(n = 8) were presented choice females of b
g8and ci (n = 16; eight for each strain) either under the free-swimming conditions, in flasks, or in holed flasks.